In this next lab that we are doing in class, we will be examining how to use micorarray analysis. If a person wanted to find changes in transcription in a specific tissue, they would use this process. One microarray can contain over 30,000 "spots of DNA" where each spot represents a different gene. The first step is to make a DNA chip, that is arranged in a pattern and that represents part of a genome. The chip could then be used to analyze complementary DNAs that were made from mRNA isolated from the same tissue. The two samples of tissue are dyed and then applied to the prepared "chip". You can determine the extent of transcrition by observing how each dyed sample adheres to its complement. Once you find this out computer analysis will reveal what similarities the two samples of tissue could have. The analysis allows scientists and doctors to find problems in certain tissues much earlier than ever before.
We will be studying six genes in this lab:
C4BPA- helps initiate part of our immune system to kill pathogens
ODC1- codes for an enzyme in the polyamine biosyntheis pathway
FGG- encoded in this gene is a part of fibrinogen, a protein found in the blood, creates blood clots
HBG1- expressed in liver, spleen, and bone marrow
SIAT9- catalyzes the formation of a protein called GM3
CYP24- catalyze many reactions involved in drug metabolism and the making of cholesterol, steroids and other lipids
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