I Shared Too Much

At the conclusion of our lab we found that two people in our class were infected with a "disease" that they had unknowingly spread to their classmates after exchanging "bodily fluids". In our lab we began by taking a sample from everyone (these were pre-made by Mr. Chugh) and exchanging them by gently mixing the samples with three people besides ourselves. When we came back the next day after storing the samples at 4 degrees celsius overnight, the found each of our samples.
We loaded each sample into two wells and then added two wells of each positive and negative control to compare our results to. After waitng four minutes we were able to "wash" the samples out with a wash buffer.Then we transferred 50 microliters of antibodies into each well so that the antigens could bind to them. After waiting 4 minutes we washed the samples two more times. After the washing we added 50 microliters of secondary antibody and then waited 4 minutes again, following with three more washes. Lastly we added 50 microliters of enzyme substrate into the twelve wells, this made the contaminated samples turn blue. At our lab table both Elizabeth's and mine were a strong blue, while Ayan and Nate were not contaminated. After surveying the class's results we were able to determine that Taylor Grey and Chloe Krey were the original holders of the "disease.

Sources of error: I shared with four people. My last person being Michael who had just shared with Chloe, leading to my contamination!