In this lab we will be comparing the muscle proteins of many different kinds of fish, determining how closely they are related. We will use protein gel electrophoresis to compare their "fingerprints". By looking at the gel we will be able to determine identify similarities and differences in the fish and their ancestors, or similar species. Each band in the gel will identify a shared characteristic with another type of fish. The muscle proteins of a fish are made up of actin and myosin, along with numerous other muscle tissues.
In this lab we will be grinding up or denaturing the muscle proteins of six different species by putting them in a buffer and then putting them into a 95 degree water bath to denature the proteins, actin and myosin. We will then run the samples on a gel and return the next day to examine the results.
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